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scc15 human squamous carcinoma cells  (ATCC)


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    ATCC scc15 human squamous carcinoma cells
    Scc15 Human Squamous Carcinoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 649 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/scc15+human+squamous+carcinoma+cells/SCC-15/pm41330921-408-0-5
    Average 96 stars, based on 649 article reviews
    scc15 human squamous carcinoma cells - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Cell Culture:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Immunofluorescence:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Expressing:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Transmission Assay:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Electron Microscopy:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Labeling:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Transwell Chamber Assay:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Migration:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Fluorescence:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Co-Culture Assay:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Transfection:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Phospho-proteomics:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Control:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Knock-Out:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    RNA Sequencing:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Staining:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Flow Cytometry:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Activity Assay:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Isolation:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    CCK-8 Assay:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Injection:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Immunohistochemical staining:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Marker:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Protein Concentration:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Mass Spectrometry:

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness.
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.

    Article Title: CAV2-expressing nerves induce metabolic switch toward mitochondrial oxidative phosphorylation to promote cancer stemness
    Article Snippet: SCC15 human squamous carcinoma cells (ATCC, CRL-1623), PC-12 rat pheochromocytoma cells (National Collection of Authenticated Cell Cultures, TCR-9), and murine oral carcinoma cell lines MOC1 (BFN6021632) and MOC2 (BFN6021637) (BLUEFBIO) were used.. SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.SCC15 cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, and 0.5 mM sodium pyruvate, supplemented with 400 ng/mL hydrocortisone and 10% fetal bovine serum (FBS), according to ATCC.



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    7SK inhibits tumor progression in vitro and in vivo . (A) Efficiency of 7SK knockdown in SCC15 cells, n = 3. (B–E) Apoptosis assay detected by Annexin V/PI staining (B) , MTS assay (C) , wound healing assay (D) , and transwell assay (E) in 7SK knockdown SCC15 cells, n = 3. For (C–E) , the representative images and corresponding statistical analysis are shown. The scale bar is 100 μm. (F) Images of nude mice injected with control or sh7SK-1 SCC15 cells, as well as the lumps from the injection of sh7SK-1 SCC15 cells. (G) HE staining of lumps from sh7SK-1 SCC15 cells injection, n = 4. The scale bar is 50 μm. Data represent the mean ± SEM. * P < 0.05. ** P < 0.01.

    Journal: Frontiers in Genetics

    Article Title: 7SK Acts as an Anti-tumor Factor in Tongue Squamous Cell Carcinoma

    doi: 10.3389/fgene.2021.642969

    Figure Lengend Snippet: 7SK inhibits tumor progression in vitro and in vivo . (A) Efficiency of 7SK knockdown in SCC15 cells, n = 3. (B–E) Apoptosis assay detected by Annexin V/PI staining (B) , MTS assay (C) , wound healing assay (D) , and transwell assay (E) in 7SK knockdown SCC15 cells, n = 3. For (C–E) , the representative images and corresponding statistical analysis are shown. The scale bar is 100 μm. (F) Images of nude mice injected with control or sh7SK-1 SCC15 cells, as well as the lumps from the injection of sh7SK-1 SCC15 cells. (G) HE staining of lumps from sh7SK-1 SCC15 cells injection, n = 4. The scale bar is 50 μm. Data represent the mean ± SEM. * P < 0.05. ** P < 0.01.

    Article Snippet: Human tongue squamous cell carcinoma cell line SCC15 was purchased from the American Type Culture Collection (ATCC).

    Techniques: In Vitro, In Vivo, Knockdown, Apoptosis Assay, Staining, MTS Assay, Wound Healing Assay, Transwell Assay, Injection, Control

    Identification of FOXJ3 and THRA as two transcription factors that oppositely regulate 7SK associated genes and SCC15 migration. (A) The top five motifs (including FOXJ3 and THRA ) identified on the upstream regulatory regions of the 27 7SK associated genes. (B) Motif distributions on the upstream regulatory regions of the 27 7SK-associated genes. (C,D) RT-qPCR of FOXJ3 , THRA , 7SK, and the 27 7SK-associated genes in the presence of siFOXJ3 (C) and siTHRA (D) treated SCC15 cells. (E,F) The representative images (left) and the according statistical analysis (right) of transwell assay after FOXJ3 (E) and THRA (F) knockdown. The scale bar is 100 μm, n = 3. * P < 0.05.

    Journal: Frontiers in Genetics

    Article Title: 7SK Acts as an Anti-tumor Factor in Tongue Squamous Cell Carcinoma

    doi: 10.3389/fgene.2021.642969

    Figure Lengend Snippet: Identification of FOXJ3 and THRA as two transcription factors that oppositely regulate 7SK associated genes and SCC15 migration. (A) The top five motifs (including FOXJ3 and THRA ) identified on the upstream regulatory regions of the 27 7SK associated genes. (B) Motif distributions on the upstream regulatory regions of the 27 7SK-associated genes. (C,D) RT-qPCR of FOXJ3 , THRA , 7SK, and the 27 7SK-associated genes in the presence of siFOXJ3 (C) and siTHRA (D) treated SCC15 cells. (E,F) The representative images (left) and the according statistical analysis (right) of transwell assay after FOXJ3 (E) and THRA (F) knockdown. The scale bar is 100 μm, n = 3. * P < 0.05.

    Article Snippet: Human tongue squamous cell carcinoma cell line SCC15 was purchased from the American Type Culture Collection (ATCC).

    Techniques: Migration, Quantitative RT-PCR, Transwell Assay, Knockdown

    Effects of indicated compounds on the cell viability of the SCC15 cells. a Exponentially growing of SCC15 cells was treated with aloe-emodin, rhein and physcion in various concentrations (0, 12.5, 25, 50, 100 and 200 μM) for 48 h. b SCC15 cells were treated with 12.5, 25, 50, 100 and 200 μM of aloe-emodin for various times (24 h, 48 h, 72 h). The cell viability was determined using MTT assay. Each bar represents the mean ± SD

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Aloe-emodin induces apoptosis in human oral squamous cell carcinoma SCC15 cells

    doi: 10.1186/s12906-018-2353-z

    Figure Lengend Snippet: Effects of indicated compounds on the cell viability of the SCC15 cells. a Exponentially growing of SCC15 cells was treated with aloe-emodin, rhein and physcion in various concentrations (0, 12.5, 25, 50, 100 and 200 μM) for 48 h. b SCC15 cells were treated with 12.5, 25, 50, 100 and 200 μM of aloe-emodin for various times (24 h, 48 h, 72 h). The cell viability was determined using MTT assay. Each bar represents the mean ± SD

    Article Snippet: Human oral squamous cell carcinoma cell line SCC15 was donated by Zhang Xin-yan, professor of Capital medical university school of stomatology (Beijing, China), which was obtained from the American Type Culture Collection (Manassas VA) and stored in our laboratory.

    Techniques: MTT Assay

    Aloe-emodin induces apoptosis of the SCC15 cells. SCC15 cells were treated by aloe-emodin of 0 μM ( a and c ) and 50 uM ( b and d ) for 24 h and 48 h, respectively. Annexin binding and propidium iodide (PI) staining were analyzed by FACScan. Q4: viable cells; Q3: early apoptotic cells; Q2: late apoptotic cells; Q1: death cells. The graph visualizes early and late stage apoptotic cells ( e ). Results were expressed as the mean ± SD ( n = 3) and analyzed by Students’ t -test ( *P < 0.05 , **P < 0.01)

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Aloe-emodin induces apoptosis in human oral squamous cell carcinoma SCC15 cells

    doi: 10.1186/s12906-018-2353-z

    Figure Lengend Snippet: Aloe-emodin induces apoptosis of the SCC15 cells. SCC15 cells were treated by aloe-emodin of 0 μM ( a and c ) and 50 uM ( b and d ) for 24 h and 48 h, respectively. Annexin binding and propidium iodide (PI) staining were analyzed by FACScan. Q4: viable cells; Q3: early apoptotic cells; Q2: late apoptotic cells; Q1: death cells. The graph visualizes early and late stage apoptotic cells ( e ). Results were expressed as the mean ± SD ( n = 3) and analyzed by Students’ t -test ( *P < 0.05 , **P < 0.01)

    Article Snippet: Human oral squamous cell carcinoma cell line SCC15 was donated by Zhang Xin-yan, professor of Capital medical university school of stomatology (Beijing, China), which was obtained from the American Type Culture Collection (Manassas VA) and stored in our laboratory.

    Techniques: Binding Assay, Staining

    Western blotting analyses of aloe-emodin on the expression of apoptotic proteins. a SCC15 cells were treatment with various concentrations (0, 25 and 50 μM) of aloe-emodin for 48 h and the levels of caspase-9, caspase-3 were measured by Western blotting analysis. Increased expression of caspase-9 and caspase-3 were observed. β-actin was used as a loading control. b Densitometry analysis of Western blots, Data were expressed as the mean ± SD ( n = 3) and analyzed by one-way ANOVA using Dunnett’s multiple-comparison test ( *P < 0.05 , **P < 0.01)

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Aloe-emodin induces apoptosis in human oral squamous cell carcinoma SCC15 cells

    doi: 10.1186/s12906-018-2353-z

    Figure Lengend Snippet: Western blotting analyses of aloe-emodin on the expression of apoptotic proteins. a SCC15 cells were treatment with various concentrations (0, 25 and 50 μM) of aloe-emodin for 48 h and the levels of caspase-9, caspase-3 were measured by Western blotting analysis. Increased expression of caspase-9 and caspase-3 were observed. β-actin was used as a loading control. b Densitometry analysis of Western blots, Data were expressed as the mean ± SD ( n = 3) and analyzed by one-way ANOVA using Dunnett’s multiple-comparison test ( *P < 0.05 , **P < 0.01)

    Article Snippet: Human oral squamous cell carcinoma cell line SCC15 was donated by Zhang Xin-yan, professor of Capital medical university school of stomatology (Beijing, China), which was obtained from the American Type Culture Collection (Manassas VA) and stored in our laboratory.

    Techniques: Western Blot, Expressing, Control, Comparison